Fig. 6
From: A novel in-vitro expression assay by LC/MS/MS enables multi-antigen mRNA vaccine characterization
Effect of Various Buffers on Phuket Monovalent Flu LNP Expression. Antigen expression in different buffers was measured by IVE-LC/MS/MS. HEK293T cells were dosed with a monovalent mRNA vaccine at four different doses. The figure depicts overlays of % antigen peptides signal to HEK293T peptides signal vs. total mRNA (ng) dosed per well for three buffer combinations. n = 2 for each transfection dose. R2 for Buffers 1, 2 and 3 are 0.993, 0.981 and 0.996 respectively.
