Fig. 7
From: A novel in-vitro expression assay by LC/MS/MS enables multi-antigen mRNA vaccine characterization
Antigen Expression is diminished after LNP shaking. Antigen expression of LNPs containing monovalent mRNA was measured after shaking for 30 and 240 min at mRNA concentrations 0.12, 0.06, and 0.01 mg/mL using IVE-flow cytometry (% HA positive cell, panels A, B, and C, and MFI ratio, panels D, E, and D) and IVE-LC/MS/MS (% Antigen/HEK, panels G, H, and I). Black squares depict non-shaken, green triangles and red diamonds depict 30- and 240-min shaking, respectively. R2 for NS, 30 min and 240 min conditions respectively are as follows: D, 0.98, 0.99, 0.99; E, 0,95, 0,95, 0,99; F, 0.98, 0.98, 0.98; G, 0.98, 0.99, 0.98; H, 0.99, 0.96, 0.99; I, 0.99, 0.98, 0.99. n = 2 for IVE-flow cytometry dose–response data.
