Fig. 8
From: A novel in-vitro expression assay by LC/MS/MS enables multi-antigen mRNA vaccine characterization
Relative levels of antigen compared to non-shaking. Antigen expression of LNPs containing monovalent mRNA was measured after shaking for 30 and 240 min at mRNA concentrations 0.12, 0.06, and 0.01 mg/mL using IVE-flow cytometry (% HA positive cell, panel A, and MFI ratio, panel B) and IVE-LC/MS/MS (% Antigen/HEK, panel C). Data represented as % change from non-shaking condition (NS). Change from NS is calculated based on average of 3 doses (IVE-MS: 50,100,150 ng/well; Flow cytometry: 8, 16, 32 ng/well).
