Fig. 1 | Scientific Reports

Fig. 1

From: PKD1 mutation perturbs morphogenesis in tubular epithelial organoids derived from human pluripotent stem cells

Fig. 1

UB spheroid differentiation from hiPSCs. (A) Overview of UB spheroid differentiation protocol. (B) Time course brightfield images of UB spheroid differentiation. Scale bar, 100 μm. (C) Gene expression analysis showing the specification of UB spheroids over 10 days. Expression levels are relative to HPRT1, n = 3 independent experiments. Data represented as mean \(\:\pm\:\) SD. (D) Wholemount confocal immunofluorescence images of day 10 UB spheroids showing expression of the ureteric bud markers HOXB7, AQP2, PAX2, CDH1 and GATA3. Scale bar, 50 μm.

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