Table 2 Details of primer for relative quantitative real-time PCR.
Gene | Primer sequence (5′ − 3′) | Accession number | Product length | Annealing temperature |
|---|---|---|---|---|
HSP 70 | F- CTCCTCCTAAACCCCGAGTC R- CCACCAGCACGTTAAACACA | XM_026934573.2 | 106 | 60 |
iNOS | F-ACACCACGGAGTGTGTTCGT R-GGATGCATGGGACGTTGCTG | XM_026931613.2 | 119 | 61 |
TNFα | F-TGGAGTTCTGCTTGCCGTGG R-GCAGCCTTTGCAGTCTCGGA | XM_026942329.2 | 141 | 62 |
Ghr1 | F-TATTGGCTACAGCTCGCCGC R-AATCACCCCGACTGTGCTGC | XM_034306157.1 | 122 | 62 |
Ig | F- GGCCAGTAATCGTACCTCCA R- CTTCGTAAGGTCCCCACTGA | XM_026923540.2 | 139 | 59 |
MYST | F-GGGAAAGACCTGGCCGTGAC R-TCGAGGCCGGATTCTCGTCT | XM_026910492.2 | 113 | 62 |
GH | F-CCCAGCAAGAACCTCGGCAA R-GCGGAGCCAGAGAGTCGTTC | GQ859589.1 | 133 | 62 |
CYP P450 | F-GATTCGGCATCCGTGCGTGC R-CGATGTGGCTGGGACGAGCA | NC_047599.1 | 155 | 60 |
Cas 3a | F-CCGGCATGAACCAGCGCAAC R-TCCACCGCACCATCTGTCCC | NC_047622.1 | 229 | 62 |
β-Actin | F-CAGCAAGCAGGAGTACGATG R-TGTGTGGTGTGTGGTTGTTTTG | XM_026929614.3 | 136 | 58 |
