Fig. 2

Differentially expressed genes (DEGs) in NASH vs. NAFL liver tissues, PPI network and functional enrichment analysis of DEGs. (A) volcano plot of all DEGs. (B) Functional PPI network, colors represent node correlativity degree evaluate by Cytoscape software. Redder colors represent higher interaction scores, while bluer colors indicate lower interaction scores. (C) Gene Ontology enrichment analysis, categorized into three domains: BP (Biological Process), MF (Molecular Function), and CC (Cellular Component). (D) KEGG enrichment analysis. (E) Gene Set Enrichment Analysis. Pathways enriched in the NAFL group are explicitly marked with an asterisk (*) on the top-right corner.