Fig. 6

AP-1 expression was reduced by NAIMS treatment. AP-1-luc reporter was transiently transfected in HeLa cells, which were then treated with 5 µM of 4c, 4e, and 4k, or XMD8-92. mRNA expression of AP-1 was checked using RT-PCR analysis with GAPDH serving as the control. Data show the mean expression ± SD of three independent experiments, calculated using the 2-ΔΔCq relative quantitation method. ##p < 0.01 vs. Control, *p < 0.05 vs. XMD8-92.