Fig. 1

(adapted from Matthias et al.30), and soil samples from the substrate directly under their corners and center. We also collected soil samples from random locations within each site > 20 m apart from each other and each ACO. The second inset shows the location of the Canary Islands and the island of Gran Canaria within the archipelago, as well the location of the study area (write cross) and Lampropeltis californiae accumulated captures and sightings from 2009 to 2024 according to the control program33 (black dots).
Map of the study area showing the location of our sampling sites and artificial cover objects (ACOs). We visited ACOs to collect swab samples following the patterns described in the top inset