Fig. 2 | Scientific Reports

Fig. 2

From: Validation of eDNA methods for managing the terrestrial invasive snake Lampropeltis californiae on the Canary Islands

Fig. 2

Specificity of the self-designed primers for the cytochrome oxidase I subunit. Panel A shows the amplification products (≈ 161 bp) obtained from the extracted DNA of Lampropeltis californiae, Gallotia stehlini, Chalcides sexlineatus and Tarentola boettgeri and visualized through 1.7% agarose gel electrophoresis with Real-Safe (Real Biotech Corporation, Taiwan) (the third-last and last lanes represent the negative controls (-C) and the second-last lane included the 25–700 bp DNA marker (LADD-DN1-500; OXGEN™). Panel B shows the melting temperature curves obtained through qPCR when amplifying the extracted DNA from tails of the four species.

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