Fig. 2

PCYT2 regulates ovarian cancer cell invasion and metastasis. This figure presents several graphs, where SKOV3/OVCAR3/control denotes the untreated cell line, and OE indicates the SKOV3/OVCAR3 cell line that overexpresses the PCYT2 gene, OE-CTR is SKOV3/OVCAR3 cell line transfected with control plasmid corresponding to the overexpression plasmid, SH refers to SKOV3 /OVCAR3 cell line knocked out of the PCYT2 gene and SH-CTR refers to SKOV3/OVCAR3 cell line transfected with control plasmid corresponding to the silence-expression plasmid. The figure displays healing area maps of cell scratches taken at various time points. 0 H represents the cell scratch area at time point 0 h, while 24 H indicates the area after 24 h of cell culture after scratched. 48 H is the cell scratch area taken again after 48 h of cell culture of the scratched. (A) Shows the healing of cell scratches in SKOV3 cells at 0, 24, and 48 h after PCYT2 overexpression and knockdown, compared to the blank and control groups. (C) Shows the healing of cell scratches in OVCAR3 cells at 0 h, 24 h, and 48 h following the overexpression and knockdown of PCYT2, compared to the blank and control groups. (E) and (F) how a transwell experiment result for SKOV3 and OVCAR3 cells that either overexpress or have knocked down PCYT2 expression. (B) and (D) present statistical plots illustrating the scratch healing ratios of SKOV3 and OVCAR3 cells before and after adjusting PCYT2 expression, respectively. The x-axis shows the different sub-groups and the y-axis shows the ratio of healed scratches. Yellow bars represent the percentage of the scratch-healed area of cells at 24 h, while grey bars represent the percentage at 48 h. (G) Illustrates the statistical differences in invasion ability between SKOV3 and OVCAR3 cells after PCYT2 regulation. The x-axis represents the different subgroups. The y-axis represents the number of cells invaded. The earthy yellow bar represents the number of infiltrating OVCAR3 cells, while the green bar indicates the number of infiltrating SKOV3 cells.