Table 5 Parameter settings for the computer-assisted semen analysis.
Parameter | Setting |
|---|---|
Frame rate | 60-Hz |
Number of frames | 30 |
Duration of capture | 1 s |
Stage temperature rate | 37 °C |
Minimum cell size | 4 pixels |
Cell size | 13 pixels |
Minimum contrast | 30 |
Cell intensity | 75 pixels |
Chamber type | Slide-Coverslip (22*22 mm) |
Volume per slide | 7 µL |
Chamber depth | ≈ 20 μm |
Minimum number of field analysis | 500 cells |
Image type | Phased contrast |
Average path velocity (VAP) | 50.0 μm/sec |
Straightness ( | 50% |
VAP cutoff | 10.0 μm/sec |
Straight line velocity cutoff | 0.0 μm/sec |
Curvilinear velocity | > 180 μm/sec |
Amplitude of lateral head | > 9.5 μm |
Mean linearity | < 38% |
