Fig. 1
From: Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle
(A) Zona-free oocytes exhibiting induced protrusion cords; (B) Cytoplast generated using the microblade method; (C) Cytoplast produced via the micropipette method, exhibiting a larger size than those obtained using the microblade method; (D) Double cytoplast aligned with a somatic cell before electrofusion; (E) Mass electrofusion: five pairs of cytoplasts aligned for electrofusion. Scale bar = 100 μm.
