Table 2 Comparison of cloning efficiency and developmental outcomes using micropipette method with single vs. double cytoplasts.
From: Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle
Method | No. of oocytes | No. of cytoplast | Fusion rate (%) | No. of reconstructed embryos | Size of the embryos (%) | Cleavage rate (%) | Blastocyte rate (%) | Cell numbers |
|---|---|---|---|---|---|---|---|---|
Double cytoplasts (n = 6) | 185 | 170 | 91.9 ± 8.1%a | 78 | 180 ~ 190 | 83.1 ± 3.7%a | 42.3 ± 5.1%a | 103.3 ± 33.2a |
Single cytoplast (n = 3) | 99 | 92 | 92.9 ± 1.5%a | 92 | 90 ~ 95 | 84.1 ± 4.1%a | 55.3 ± 4.0%b | 85.1 ± 28.1a |
