Fig. 4
From: Accelerated vaccine process development by orthogonal protein characterization
LC/MS/MS analysis for protein identification in RP-HPLC fractions: (A) LC/MS/MS workflow schematic including digestion of protein fractions, separation by nanoUPLC followed by data dependent acquisition on a Q Exactive HF mass spectrometer. (B) UV280 nm chromatogram of purified drug substance with HPLC fractions collected by time in 30 s intervals. (C) A bar plot representation of the relative intensity of proteins detected in the fraction identification LC/MS/MS experiments. Insert, table summary of main protein identity results for HPLC fractions.
