Fig. 6
From: Accelerated vaccine process development by orthogonal protein characterization
RP-HPLC analysis of ancestral, Beta and Omicron BA.1 SARS-CoV-2 spike antigen constructs produced in baculovirus-vector expression system. Purified antigen material demonstrating high purity by RP-HPLC. Retention of main antigen peak was identical for ancestral and Beta spike antigens. Omicron BA.1 spike antigen exhibited distinctly earlier retention time by RP-HPLC, reflecting overall lower hydrophobicity arising from differences in amino acid composition.
