Table 3 Amplification system of single PCR.

From: A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli

Element

Volume (μL)

2 × Universal Green PCR Master Mix

12.5

forward primer(10 pmol/L)

1

reverse primer(10 pmol/L)

1

ddH2O

8.5

DNA

2

Total volume

25

  1. Procedure of reaction: 95 °C 5 min; 94 °C 30 s; 57 °C 30 s; 72 °C 20 s; 72 °C 8 min; 32 cycles.
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