Fig. 4
Excessive autophagosome accumulation contributes to the anti-PC effect of OST. (A) The relative absorbance at 570 nm of PANC-1 and MIA PaCa-2 cells incubated with or without 100 µM OST in the presence or absence of 5 mM 3-MA for 24 h. (B) Colony formation assay of PC cells treated with or without 100 µM OST in the presence or absence of 5 mM 3-MA. (C) The relative absorbance at 570 nm of PC cells transfected with siScramble or siAtg5 for 24 h, followed by treatment with or without 100 µM OST for another 24 h. (D, E) The relative absorbance at 570 nm of PC cells incubated with or without 100 µM OST in the presence or absence of 10 µM CQ or 100 nM Baf-A1 for 24 h. (F) Colony formation assay of PC cells treated with or without OST in the presence or absence of 10 µM CQ. (G) Western blot analysis of cleaved PARP and cleaved caspase 3 expression in PC cells incubated with or without 100 µM OST in the presence or absence of 5 mM 3-MA for 24 h. (H) Western blot analysis of cleaved PARP and cleaved caspase 3 expression in PC cells incubated with or without 100 µM OST in the presence or absence of 5 mM 3-MA for 24 h.
