Fig. 4
Differential expression of genes involved in apoptosis, inflammation, and mitochondrial turnover in EAE mice with constitutive expression of Sirt1. (A-E) GSEA performed with signatures for apoptosis, inflammation (TNF-alpha signaling), unfolded protein, SIRT1 target, and mitochondrial turnover-associated genes (used with permission from Kanehisa laboratories). (F) Heatmap of mitochondria-associated genes with highest enrichment scores in WT and SIRT1 KI mice with EAE. (G-I) Differential gene expression of the Atg4b, Arfip2, and Slc25a46genes validated using qRT-PCR in WT and SIRT1 KI retinas of mice with or without EAE. P values indicate the significance compared to EAE untreated mice (n = 10). Data represented as mean ± SEM. Significance was analyzed by ANOVA of repeated measures followed by Tukey’s honest significant difference test. *p < 0.05.
