Fig. 5

The changes of fatty acids composition across developmental stages of the mammary gland. (A) Principal component analysis showing the differential distribution of the fatty acids across the groups. (B) In the heatmap of 41 fatty acids across developmental stages, the concentration of the lipids was clustered and represented in the rows, while the samples are represented in the columns from PND21, PND46, and PND90. (C) Correlation of fatty acids showing the different lipid profiles at PND21, PND46, and PND90 groups; the red color indicates a negative correlation in the interaction between lipids and the blue color indicates a positive correlation. (D) Histogram representation of all categories of fatty acids such as saturated fatty acids, total cis fatty acids, total omega-6 fatty acids, total omega-3 fatty acids, and ratio of omega-3 to omega-6 fatty acids. Error bars represent SD (*p < 0.05, **p < 0.01).