Fig. 1
From: Saponins in soy reduce NNK-induced lung cancer by increasing plasma isoflavone levels
The efficacy of SPI in reducing NNK-induced lung nodule formation in A/J mice is not due to soy isoflavones. Female A/J mice (n = 12) were acclimatized to either a 15% Amylose/30% casein/50% fat diet, a 15% Amylose/30% SPI/50% fat diet or a 15% Amylose/30% casein/50% fat + isoflavone diet for 2 weeks before IP injection of NNK (50 mg/kg body weight) twice, one week apart. The mice were then kept on their respective diets for 20 weeks, euthanized and lung tumor nodules counted by two blinded counters using a Leica MZ9.5 microscope. (A) Data shown are the biological replicates of lung nodule counts per mouse as mean ± SEM. Significant differences between treatment groups were identified using one-way ANOVA followed with Tukey’s multiple comparison test. Different letters denote significant (P < 0.05) differences between treatments. (B) Representative H&E images of the tumors in the lung. Images were taken using Olympus BX46 microscope at × 100 magnification.
