Fig. 5
From: Rubidium ions as a novel therapeutic approach for glioblastoma
Effect of mTOR activator MHY1485 on the antitumor effects of Rb⁺: rescue experiment. (A) CCK8 assay showed that mTOR activation partially reversed the Rb⁺-induced inhibition of cell proliferation. (B) Flow cytometry revealed that mTOR activation could not improve the cell cycle arrest induced by Rb⁺. (C) Apoptosis analysis showed that mTOR activation reduced apoptosis rates induced by Rb⁺. (D) Western blot analysis confirmed that MHY1485 restored the phosphorylation levels of 4EBP1 and P70S6K. The original blots are presented in Supplementary Fig.S5. The samples derive from the same experiment and that blots were processed in parallel.
