Table 1 Gene primer sequences used in qPCR.
From: Selective apoptosis induction by antimicrobial peptide TP4 in MCF-7 breast cancer cells
Gene | Primer sequence | Primer efficiency% | Accession number |
|---|---|---|---|
Bax | F:5’ GGACGAACTGGACAGTAACATGG3’ R: 5’ GCAAAGTAGAAAAGGGCGACAAC3’ | 99 | BC001901.2 |
Bcl2 | F:5’ TTGATGGGATCGTTGCCTTATGC3’ R: 5’ CAGTCTACTTCCTCTGTGATGTTG3’ | 99 | NM000633.2 |
P53 | F: 5’ TAACAGTTCCTGCATGGGCGGC3’ R: 5’ AGGACAGGCACAAACACGCACC3’ | 100 | NM_000546.5 |
Caspase3 | F: 5’ GAAGCGAATCAATGGACTCTGG3’ R: 5’ GACCGAGATGTCATTCCAGTGC3’ | 99 | NM032991 |
GAPDH | F: 5’GGTGAAGGTCGGAGTCAACG3’ R: 5’TGAAGGGGTCATTGATGGCAAC3’ | 100 | NM_001256799 |
