Fig. 4
From: Investigation of PANoptosis pathway in age-related macular degeneration triggered by Aβ1-40
WB and qPCR analysis of proteins and genes. (a) WB analysis of AIM2, PYRIN, ZBP1, ASC, GSDMD-N, CASP-1, Cle-CASP1 and NLRP3 protein levels in the retina-choroid complex (n = 3). (b) WB analysis of FADD, BAX, BCL-2, CASP-7, CASP-3, CASP-8 and protein levels in the retina-choroid complex (n = 3). (c) WB analysis of p-MLKL; MLKL; RIPK1; RIPK3 protein levels in retina-choroid complex (n = 3). (d) qPCR detection of Aim2, Zbp1 and Mefv in retina-choroid complex. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the Aβ1-40 group. NFL: Nerve fiber layer; INL: Inner nuclear layer; ONL: Outer nuclear layer.
