Fig. 7
From: Investigation of PANoptosis pathway in age-related macular degeneration triggered by Aβ1-40
Three Inhibitors of Regulated Cell Death Protect ARPE-19 Cells from Injury Induced by Aβ1-40. (a) Post-Aβ1-40 and Z-VAD treatment, WB of BAX, BCL-2, CASP-3 in ARPE-19 cells. (b) Post-Aβ1-40 and DSF treatment, Western blots of Cle-CASP1, GSDMD-N in ARPE-19 cells. (c) Post-Aβ1-40 and Nec-1 treatment, WB of p-MLKL, MLKL in ARPE-19 cells. (d) After RCD inhibitor and Aβ1-40 mix treatment, WB of BAX, caspase-3, Bcl-2, GSDMD-N, Cle-cas1, p-MLKL, MLKL in Vitro. Values are expressed as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Aβ1-40. DSF: disulfiram; Nec1: necrostatin-1; Z-VAD: Z-VAD-FMK.
