Fig. 2
Salinomycin and oxaliplatin combination treatment efficiently inhibits proliferation and induces apoptosis in CRC cells. (A) After treatment of Caco-2 and SW480 cells with DMSO, salinomycin, oxaliplatin and their combination for 48 h, cells were cultured for 2 weeks and analyzed by colony formation assay. A representative image of colony formation from three independent experiments is presented. (B) The number of colonies of various groups presented in the graphs in A were quantified. (C) CRC cells were treated for 48 h with DMSO and drugs as previously described. Detection of the apoptosis rate of CRC cells via Annexin V-FITC/PI double staining. (D) Quantitative analysis of apoptotic cells in C. (E-H) CRC cells were exposed to medium containing salinomycin and oxaliplatin for 48 h, and cell cycle arrest was detected by flow cytometry. The cell cycle arrest of various groups was quantified. (* P < 0.05, ** P < 0.01 and *** P < 0.001 versus control group; &&& P < 0.001 versus salinomycin group and $$$ P < 0.001 versus oxaliplatin group).
