Fig. 5
From: Identification and evaluation of Pharmacological enhancers of the factor VII p.Q160R variant
Effect of abexinostat on expression, secretion, and activity on mutant rFVII. CHO-K1 cells were transiently transfected with pcDNA3-FVII p.Q160R. 24 h post transfection the cells were stimulated with indicated levels of abexinostat for 24 h before harvest. F7 mRNA levels were measured with qRT-PCR using 18 S as the endogenous control gene (A). FVII:Ag levels were measured with ELISA in conditioned medium (B) and cell lysates (C). FVII activity was measured in conditioned medium (D). All values were normalized against the corresponding total protein or 18 S content in the respective samples. The bars represent mean + SD from at least three independent experiments performed in duplicate or triplicate (n ≥ 6).
