Fig. 2

Exploration of the off-target sites. (A) Blastn result for the NtSPS1 sgRNA in China Tobacco Genome Database. (B) DNA sequences cognition of the 3 off-target genes among the sgRNA site. (C) There were no off-target items detected for the 4 genes in ntsps1 mutants. Ntab0214390, NtSPS1 gene.