Fig. 4 | Scientific Reports

Fig. 4

From: Gene editing, metabolomics, network pharmacology strategies to explore terpenoid content and anti-TMV activity in NtSPS1 knockout Nicotiana tabacum

Fig. 4

Untargeted metabolomics analysis. (A) LC-MS/MS-base multiple reaction monitoring mode (MRM) of one NtSPS1 knockout HD (HDK) in positive ion mode. (B) PCA loading plot of the 47 terpenoids. HDS, the wild types. Numbers are the IDs of 47 terpenoids. (C) Heatmap analysis was conducted on 10 terpenoids which change significantly between HDS and HDK, the content of terpenoids were normalized and visualized where the red and green color represented up- and down-regulation. (D) The variation in terpenoid content (Log2FC) between HDS and HDK. For each test, three specialized replicates were managed. At least 6 tobacco were evaluated in each sample. Data interpreted are mean ± SE of three specialty tests.

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