Fig. 1
From: Production of live tiger salamander offspring using cryopreserved sperm

Experimental process of freezing tiger salamander (Ambystoma tigrinum) sperm with six different cryosolution treatments. The osmolality of each treatment is shown in italics text. Diluted HAM’s F-10 is HAM’s F-10 medium diluted to approximately 90 mOsm/kg.