Table 1 Pre-freeze progressive motility, stationary motility, total motility, velocity of movement, and normal morphology of sperm in samples stored in each of the three extenders (10% Holt = 10% Holtfreter’s, HAM’s = HAM’s F-10 nutrient mix, and Dil HAM’s = diluted HAM’s to 90 mOsm) from tiger salamanders (n = 12 males).
From: Production of live tiger salamander offspring using cryopreserved sperm
Extender | Progressive motility (%) | Stationary motility (%) | Total motility (%) | Velocity (0–5) | Normal morphology (%) |
|---|---|---|---|---|---|
10% Holt | 28.8 ± 3.6 | 28.3 ± 2.2 | 57.2 ± 2.9 | 3.1 ± 0.3 | 68.6 ± 3.1 |
Dil HAM’s | 34.8 ± 3.9 | 25.6 ± 2.5 | 60.5 ± 3.2 | 3.2 ± 0.3 | 69.0 ± 2.4 |
HAM’s | 31.6 ± 3.7 | 28.1 ± 1.9 | 59.8 ± 3.2 | 3.2 ± 0.2 | 70.1 ± 2.8 |
