Table 2 Post-thaw metrics for tiger salamander (n = 12 males with 2–4 straws averaged per treatment) sperm samples frozen with combinations of the two cryoprotectants, 10% DMFA or 10% DMSO, and the three extenders, 10% Holtfreter’s (10% Holt), HAM’s F-10 nutrient mix (HAM’s), and diluted HAM’s to 90 mOsm (Dil HAM’s).

DMFA

 10% Holt

3.9 ± 0.9^c

15.7 ± 3.0^c

1.8 ± 0.3^cd

20.7 ± 2.8^b

63.8 ± 2.9^a

 Dil HAM’s

11.0 ± 3.2^ab

23.5 ± 4.9^b

2.1 ± 0.3^bc

30.6 ± 4.3^a

60.2 ± 2.8^bcd

 HAM’s

10.7 ± 2.4^ab

26.6 ± 4.8^b

2.6 ± 0.1^a

29.1 ± 4.3^a

58.6 ± 2.6^d

DMSO

 10% Holt

2.7 ± 0.7^d

16.6 ± 2.7^c

1.6 ± 0.3^d

24.3 ± 2.8^b

57.7 ± 3.3^d

 Dil HAM’s

10.0 ± 2.4^b

26.4 ± 4.5^b

2.2 ± 0.2^ac

32.0 ± 34.8^a

61.6 ± 3.1^ac

 HAM’s

12.7 ± 2.5^a

32.1 ± 5.0^a

2.3 ± 0.1^ab

33.1 ± 4.5^a

62.5 ± 3.0^ab