Fig. 3
From: A model workflow for microfluidic enrichment and genetic analysis of circulating melanoma cells
Representative images of captured melanoma cells identified by Parsortix and CellSearch systems. (A) Melanoma cells (A375-MA2) were enriched and stained with Parsortix system using melanoma markers (in green, CD146/MART-1/HMW-MAA/GP100), leukocyte and endothelial markers (in pink, CD45/CD16/CD34), and DAPI for cell nuclei (in blue). Scale bar (lower right) represents 10 μm. (B) Melanoma cells (A375-MA2) enriched and stained with CellSearch platform. Cells were immunomagnetically captured by a ferrofluid antibody against CD146, and were stained with HMW-MAA melanoma marker (MEL-PE), CD45/CD34 (APC) leukocyte and endothelial markers, respectively, and DAPI for cell nuclei. Abbreviations: APC, allophycocyanin; FITC, fluorescein isothiocyanate; MEL-FITC, melanoma markers; PE, phycoerythrin.
