Table 2 Spike-in experiments to evaluate the performance of theParsortix system.
From: A model workflow for microfluidic enrichment and genetic analysis of circulating melanoma cells
Sample Characteristics | CellSearch | Parsortix | |||||
|---|---|---|---|---|---|---|---|
Experiment ID | Sample type | Staining | Melanoma cell count | Melanoma cell count | Capture Rate (%) | Number of harvested cells | Harvest Rate (%) |
1 | SK-MEL-28 spiked in HD | Celltracker orange | ND | 15 | NA | 12 | 80 |
2 | SK-MEL-28 spiked in HD | Celltracker orange | ND | 11 | NA | 8 | 73 |
3 | SK-MEL-28 + HUVEC spiked in HD | Melanoma Ab cocktail | 9 | 15 | 167 | 11 | 73 |
4 | SK-MEL-28 + HUVEC spiked in HD | Melanoma Ab cocktail | 13 | 11 | 85 | 11 | 100 |
5 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 7 | 8 | 114 | 5 | 63 |
6 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 23 | 32 | 139 | 24 | 75 |
7 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 24 | 18 | 75 | 4 | 22 |
8 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 18 | 26 | 144 | 26 | 100 |
9 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 6 | 8 | 133 | 7 | 88 |
10 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | 18 | 13 | 72 | 7 | 54 |
11 | SK-MEL-28 spiked in HD | Melanoma Ab cocktail | ND | 276 | NA | 235 | 85 |
12 | A375-MA2 spiked in HD | Melanoma Ab cocktail | 584 | 450 | 77 | 432 | 96 |
13 | A375-MA2 spiked in HD | Melanoma Ab cocktail | 45 | 33 | 73 | 24 | 73 |
