Table 3 Harvest rate of the Parsortix system by adding 1.8% of BSA to therunning buffer.
From: A model workflow for microfluidic enrichment and genetic analysis of circulating melanoma cells
Sample Characteristics | Parsortix | ||||
|---|---|---|---|---|---|
Experiment ID | Sample type | Staining | Number of captured cells | Number of harvested cells | Harvest Rate (%) |
14 | SK-MEL-28 spiked in HD blood | Melanoma Ab cocktail | 51 | 49 | 96 |
15 | A375-MA2 spiked in HD blood | Melanoma Ab cocktail | 66 | 52 | 79 |
16 | H441 spiked in HD blood | Epithelial Ab cocktail | 72 | 61 | 85 |
17 | H441 spiked in HD blood | Epithelial Ab cocktail | 68 | 46 | 68 |
18 | SK-MEL-28 spiked in HD blood | Melanoma Ab cocktail | 76 | 73 | 96 |
19 | A375-MA2 spiked in HD blood | Melanoma Ab cocktail | 53 | 48 | 91 |
20 | A375-MA2 spiked in HD blood | Melanoma Ab cocktail | 35 | 31 | 89 |
21 | SK-MEL-28 spiked in HD blood | Melanoma Ab cocktail | 52 | 50 | 96 |
22 | H441 spiked in HD blood | Epithelial Ab cocktail | 69 | 57 | 83 |
23 | H441 spiked in HD blood | Epithelial Ab cocktail | 132 | 121 | 92 |
