Table 1 Specific primers used in the course of the study

From: Consequences of vitamin D deficiency or overdosage on follicular development and steroidogenesis in Normo and hypo calcemic mouse models

Gene

Primer sequences

Length of PCR product

TM

CYP11A1

3βHSDVI

Forward 5`-TCCTTTGAGTCCATCAGCAG-3`

Reverse 5`-GTCCTTCCAGGTCTTAGTTCT-3`

Forward 5`-TCAGAGTGGATTGGAACA-3`

Reverse 5`-GGAATTGATAAGAGTTGAGTTG-3`

179 bp

147 bp

58°c

58°c

CYP19A1

Forward 5`-AAGCACAGTCACTACATCTCC-3`

Reverse 5`-CCACACAAACTTCCACCATTC-3`

175 bp

62°c

GAPDH (Housekeeping)

Forward 5`- TGCCGCCTGGAGAAACC − 3`

Reverse 5`- TGAAGTCGCAGGAGACAACC − 3`

121 bp

60°c

Back to article page