Fig. 1
From: Reliable investigation of DNA methylation using Oxford nanopore technologies
Workflow for data analysis. (1) HCT 116 WT and IPMK KO cell culture; (2) Cell lysis and total genomic DNA extraction; (3) ONT library preparation and adapter ligation; (4) DNA library loading; (5) ONT sequencing; (6) Basecalling with 5mC methylation, and alignment; (7) Whole-genome methylation summary; (8) Concordance and bias analysis of DNA methylation.
