Fig. 3 | Scientific Reports

Fig. 3

From: Interaction between ELMO1 DNA methylation and Med31 promotes H. pylori-induced gastric cancer EMT and intestinal metaplasia via M2 polarization

Fig. 3

ELMO1 methylation promotes H. pylori-induced intestinal metaplasia in GC. (A) CCK8 assay was used to determine the cell viability of H. pylori-infected AGS cells with 5-Azacytidin treatment. (D) ELMO1 methylation was measured through MSP analysis. Representative gel images of MSP products. Lanes are as follows: 1, Marker; 2–3, Control group; 4–5, H. pylori-infected group; 6–7, H. pylori-infected + 5-Azacytidin treatment group. For each group, the left lane (M) shows the PCR product from the methylated gene, and the right lane (U) shows the product from the unmethylated gene. Quantitative analysis of ELMO1 methylation levels from three independent experiments. (E) The mRNA expression levels of DNMT1, DNMT3A, and DNMT3B were evaluated by qRT-PCR analysis. (F–G) The intestinal metaplasia-related markers (CDX2, MUC2, and KLF4) were detected by qRT-PCR and Western blotting.

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