Fig. 4
Evaluation of PR8S in vivo efficacy via intramuscular injection. ICR mice (n = 8 per group, N > 2) were intramuscularly injected with 4 µg of unmodified mRNA encoding PR8S (P) or GFP (G), together with 4–8 µg of mRNA encoding luciferase (L) denoted, respectively, as P4L4, P4L8, G4L4 and G4L8. Non-treated (NT) group was injected with buffer without mRNA. Bioluminescence was assayed by IVIS 5 h after injection and daily thereafter until signals dropped to background. (A) For each group one representative image was shown per time point until bioluminescence was undetectable. (B) Expression kinetics based on bioluminescence. Results were shown as mean \(\:\pm\:\) SEM. Experiment was independently repeated three times with the same outcome. Results from one representative experiment was shown.
