Table 1 Primers’ characteristics used in Real-time RT-PCR Reaction.

From: Identification of diagnostic and prognostic biomarkers in lung adenocarcinoma through integrated bioinformatics analysis and real time PCR validation

Parameters

CYP2C9-mRNA

A2M -mRNA

KRT14-mRNA

PECAM1-mRNA

18s rRNA

Forward primer (F Primer)

CAGAGACGACAAGCACAACCCT

GTTGAAGAGCCTCACACGGAGA

TGCCGAGGAATGGTTCTTCACC

AAGTGGAGTCCAGCCGCATATC

GTAACCCGTTGAACCCCATT

Primer length

22

22

22

22

20

Reverse primer (R Primer)

ATGTGGCTCCTGTCTTGCATGC

TTCCACTCGGTGATGGTGTCAG

GCAGCTCAATCTCCAGGTTCTG

ATGGAGCAGGACAGGTTCAGTC

CCATCCAATCGGTAGTAGCG

Primer length

22

22

22

22

20

Optimal annealing temperature

58

59

℃ 53.5

58

℃ 54.5

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