Fig. 4
Photomicrographs of liver sections stained with H&E. A & E: The control group shows normal liver architecture, with hepatocytes exhibiting central rounded vesicular nuclei. Hepatic blood sinusoids (S) are radially arranged between hepatic cords. Normal bile duct (B) is observed in the portal area (P). B & F: The HFD group shows widespread macrovesicular lipid globules (black arrows) with squeezed nuclei into the thin rim of cytoplasm in some hepatocytes, others with microvesicular lipid droplets (arrowheads). Inflammatory cellular infiltration (curved arrows) appears near congested and dilated portal veins (P) and blood vessels (c) with slightly dilated blood sinusoids (S). Some hepatocytes exhibit cytoplasmic vacuolation with pyknotic nuclei (Doted arrow). C & G: The HFD + VD group shows normal hepatocytes (black arrow), and few cells show moderate microvesicular lipid droplets (arrowheads). Normal appearance of portal vein (P), mild dilated blood sinusoids (S) and mild enlarged blood vessels (c) can be seen. D & H: HFD + IF displays a normal structural pattern of tissue and a normal appearance of hepatocytes radiating around a central vein (CV); some cells appear binucleated (black arrows). Normal blood sinusoids (S) and blood vessels (c) can be noticed. [A, B,C & D: x100, E, F,G & H: x400, Scale bar; 50 μm].
