Fig. 1

PAB inhibits the proliferation of lung cancer cells. (A) The viability of A549, H460 and HBE cells is measured by CCK8 assays following treatment with PAB (0, 2.5, 5, 10, 20, 40 µM) for 72 h. (B) The cell viability of A549 and H460 treated with PAB (0, 2.5, 5, 10, 20, 40 µM) for 72 h is detected by crystal violet staining. (C) The quantitative analysis of cell death of the A549 and H460 cells is measured by the treatment with PAB (0, 2.5, 5, 10, 20, 40 µM) for 72 h.(D) Representative Edu assay showing proliferation analysis of A549 and H460 after PAB treatment for 16 h. Hoechst 33342 is used as a nuclear counterstain. Scale bars, 50 μm. The results are presented as the means ± S.D., n ≥ 3, *** p < 0.001 versus control group; ns, no significance.