Table 7 Representative transcriptional fold-change values and corresponding ciprofloxacin MIC changes following PAβN exposure in selected MDR Klebsiella pneumoniae isolates.

From: Integrated analysis of global regulators and efflux genes associated with antimicrobial resistance reversal in multidrug resistant Klebsiella pneumoniae

Isolate ID

marA (FC)

soxS (FC)

rob (FC)

acrB (FC)

Ciprofloxacin MIC (µg/mL)

MIC + PAβN (µg/mL)

MIC Reduction (fold)

KP-01

5.78

4.53

1.83

7.89

64

8

8

KP-02

5.78

4.53

1.83

7.89

32

4

8

KP-03

5.78

4.53

1.83

7.89

64

4

16

KP-04

6.12

4.74

1.93

8.46

16

4

4

KP-05

6.12

4.74

1.93

8.46

64

8

8

KP-06

5.45

4.21

1.72

7.55

16

8

2

KP-07

6.05

4.66

1.88

8.23

32

4

8

KP-08

5.12

3.98

1.62

6.92

8

4

2

KP-09

6.30

4.89

1.97

8.60

64

4

16

KP-10

5.34

4.11

1.70

7.31

16

8

2

  1. Fold-change (FC) values shown in Table 7 are directly extracted from the complete raw qRT-PCR dataset provided in Supplementary File S1 and were calculated using the 2⁻ΔΔCt method without rounding or modification. The table includes selected MDR isolates subjected to PAβN-based efflux inhibition assays to illustrate the relationship between efflux gene expression and ciprofloxacin MIC reduction. PAβN was used at a final concentration of 25 µg/mL. A ≥ fourfold decrease in MIC was interpreted as indicative of significant efflux pump contribution.
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