Fig. 2
Deficiency of TRMT6 in intestinal stem cells aggravated colitis in mice. (A) Schematic diagram of DSS-induced mouse colitis model in Trmt6Flox/Flox Lgr5creERT and Trmt6Flox/Flox mice. (B) Western blot analysis for assessing TRMT6 knockout efficiency. (C) Body weight following DSS treatment in Trmt6Flox/Flox Lgr5creERT and Trmt6Flox/Flox mice. (D) Gross morphology and length of colon from Trmt6Flox/Flox Lgr5creERT and Trmt6Flox/Flox mice treated with DSS. (E) H&E staining of small intestine in DSS-induced Trmt6Flox/Flox Lgr5creERT and Trmt6Flox/Flox mice. (F) H&E histological scores of small intestine in Trmt6Flox/Flox Lgr5creERT and Trmt6Flox/Flox mice treated with DSS. Data are presented as the mean ± SD from three independent experiments. Statistical significance for body weight was determined using multiple unpaired t-tests, while unpaired two-tailed parametric t-tests were applied to analyze all statistical data shown in the above bar graphs. Significance levels are denoted as follows: ***, P < 0.001. Original blots and gels are provided in the Supplementary Material 1.
