Fig. 3
From: Targeting the Akt–EphA2 axis and cell–cell adhesion enhances anoikis sensitivity in cancer cells
KEGG pathway enrichment analyses for MDA-MB-231 cancer cells induced by Tpm2.1 overexpression and suspension growth conditions. (A) KEGG pathway enrichment analysis of upregulated and downregulated genes in MDA-EV, Tpm_NoAp, and Tpm_Apop cells after 5 h and 4 days in suspension, relative to their respective cell populations cultured under attached (rigid) conditions used as controls (MDA-EV, and MDA-Tpm2.1, respectively). Red bars represent upregulated genes. Green bars represent downregulated genes. All pathways have adjusted p < 0.05, |log2FoldChange| > 1. (B,C) Heatmaps of differentially expressed genes from KEGG analysis for the Cell Adhesion Molecules (CAM) pathway (B) and the PI3K-Akt signaling pathway (C). Expression levels are color-coded, with red indicating high expression and green indicating low expression. Heatmaps were generated using an R script, with adjusted p-value (padj) < 0.05 and |log2FoldChange| > 1, applying Pearson correlation and the centroid method for clustering.
