Fig. 4

Change in cell-cell interaction affects anoikis in MDA-Tpm2.1 cells. (A) Phase-contrast images of MDA-EV and MDA-Tpm2.1 cells cultured at three different cell densities for 4 days in suspension. MDA-EV cells are indicated by arrows, MDA-Tpm2.1 cell aggregates are highlighted by circles. Scale bar = 750 μm (B) Mean ± SD of total apoptotic cells (Annexin V⁺ only, PI⁺ only, and Annexin V⁺/PI⁺; %) in suspension cultures at different cell densities for 4 days. Statistical significance was determined using two-way ANOVA followed by Šídák’s multiple comparisons test. (C,D) Mean ± SD of total apoptotic cells (Annexin V⁺ only, PI⁺ only, and Annexin V⁺/PI⁺; %) in MDA-EV (C) and MDA-Tpm2.1 (D) cells treated with ICAM-1 inhibitor (ICAM-1-IN-1, 100nM) for 1 day or 4 days under suspension and attached conditions. The ICAM-1 inhibitor was added at the time of seeding. Statistical significance was determined using unpaired t-test followed by Welch’s correction and Holm-Šídák adjustment for multiple comparisons (α = 0.05). (**) for p value < 0.01, (***) for p value < 0.001, (****) for p value <0.0001.