Fig. 4

LanaC reduces DSS-induced macrophage infiltration and modulates macrophage polarization. (A) The proportion of macrophages (CD11b⁺F4/80⁺) in the colon tissues were assessed by flow cytometry; (B) The proportion of macrophages (CD11b⁺F4/80⁺) in the spleen were assessed by flow cytometry; (C) The percentage of iNOS⁺ or CD206⁺ macrophages in the spleen and colon tissues were determined by flow cytometry; (D) RT-PCR determination of iNOS, CD80, Arg1, and CD206 mRNA levels in colon tissue; All experiments were performed with 6 mice per group and presented as means ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.