Fig. 1

Integration and annotation of cellular subpopulations in tumor and background HB samples. a. UMAP plot showing successful integration of 17 background and tumor samples, colored by sample ID. b. UMAP colored by 11 broad cell types. c. Same UMAP as panel B but split to show only background (left) and tumor (right) samples. d. Dot plot showing average normalized expression (color) and the percent of cells expressing the gene (dot size) for established lineage and cluster markers. e. Stacked proportional bar plot showing the prevalence of each cluster within the background (left) and tumor (right) samples. All clusters add up to 100% for each condition. f. Box plots for the Hepatocyte (left), Cycling Hepatocyte (middle), and Cholangiocyte-like (right) clusters showing decreased prevalence in tumor for Hepatocyte (p = 0.00032), and increased prevalence in tumor for Cycling Hepatocyte (p = 0.027) and Cholangiocyte-like (p = 0.00065) clusters. A Wilcoxon test was used for statistical analyses.