Fig. 3

Characterization of MTX@DXM-MN bilayer microneedles. (A) Dual-layer fluorescence image of MTX@DXM-MN. (B) Full image display of MTX@DXM-MN. (C) SEM image of MTX@DXM-MN, scale bars: 30, 200 and 100 μm. (D) Mechanical testing of MTX@DXM-MN. (E) Penetration force of MTX@DXM-MN into skin. (F) Fluorescence microscopic image of Rho B-labeled MTX@DXM-MN penetrating mouse skin. (G-H) In vitro drug release curves of MTX@DXM-MN. Data represent three independent experiments.