Fig. 4
The inhibitory effect of MTX@DXM-MN on TNF-α-induced HaCaT cells in vitro. (A)Cell viability after 48 h after co-culture of various patch with HaCaT cells. (B) Calcein-AM/PI staining results after co-culture of various patch with HaCaT cells. Scale bar: 50 μm. (C) Hemolysis test of different concentrations of MTX@DXM-MN. The positive control was water, and the negative control was PBS. (D-E) Representative EDU cell images and positive value-added rate after 3 days of co-culture of various patch with HaCaT cells. Scale bar: 50 μm. (F-G) Representative HaCaT cell images and migration rate after 24 h of co-culture of various patch with HaCaT cells. Scale bar: 50 μm. Data is presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. One-way ANOVA with Tukey’s multiple comparisons test in (E and G). Scale bars are indicated. Data are representative of three independent experiments.
