Fig. 5 | Scientific Reports

Fig. 5

From: A bilayered microneedle patch loaded with methotrexate and dexamethasone for transdermal treatment of psoriasis

Fig. 5The alternative text for this image may have been generated using AI.

MTX@DXM-MN promotes the polarization of RAW264.7 cells towards the M2 phenotype thereby facilitating inflammation regulation. (A-B) Representative immunofluorescence images illustrating macrophage polarization markers. CD206 serves as an M2 macrophage marker, while CD86 represents the M1 macrophage marker. (C-F) Evaluation of the relative mRNA expression levels of TNF-α (C), Arg-1 (D), CD86 (E) and CD206 (F) in RAW264.7 cells under different conditions. (G-J) ELISA detection of TNF-α, IL-6, IL-1β, and IL-17 A expression in the supernatant of RAW24.7 cells from each group. Data is presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. One-way ANOVA with Tukey’s multiple comparisons test in (C-F). (G-J) analyzed using one-way ANOVA with Tukey’s post-hoc test. Scale bars are indicated. Data are representative of three independent experiments.

Back to article page